Frequently asked questions from our customers

Genetic Background Testing

Scientific Questions
Why is it necessary to determine the genetic background?

A clearly defined genetic background is essential for the reproducibility of studies with genetically modified lines. Inbred strains have accumulated different mutations that can have a strong influence on the phenotype of the test animals. Substrains of C57BL/6 with identical designation, but provided by different breeders, differ genetically significantly. Knowing which inbred strain is specifically used is necessary so that the correct reference strain can be used in a study.

When generating a genetically modified mouse line, different strains are often included. By appropriate breeding measures, they must subsequently be backcrossed to a defined genetic background. The identity of the inbred strain and the degree of admixture of foreign DNA can be checked by determining the genetic background.

Genetic quality assurance and genetic monitoring of laboratory mice and rats:
FELASA Working Group Report (Laboratory Animals, vol. 2 2020):

  • Verify authenticity and uniformity of inbred strains and substrains
  • Describe precise genetic composition of genetically modified lines including Y chromosomal origin
  • Detect potential genetic contamination
  • Monitor genetic drift
  • Obtain qualified breeding advice to speed up congenic backcrossing projects
  • Quantify and control genetic diversity of outbred strains
Technical Questions
What is the turnaround time per sample?

Turnaround time is maximum 2 weeks (10 working days). However, most of the times it is shorter.

Do you have any further questions?

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